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B3150
Sigma
Monoclonal Anti-Protein A−Biotin antibody produced in mouse
clone SPA-27, purified immunoglobulin, buffered aqueous solution
| Synonym: | Monoclonal Anti-Protein A |
Description
| Immunogen | protein A from Cowan I strain of Staphylococcus aureus. |
| Preparation Note | Prepared by conjugation of biotinamidocaproate N-hydroxysuccinimide ester to fractionated monoclonal anti-Protein A. |
| Specificity | Specific for Protein A and shows no cross-reactivity with Protein G. Reacts with free Protein A or IgG bound Protein A and does not interfere with the Fc binding activity of Protein A. |
| Physical form | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% inactivated bovine serum albumin and 15 mM sodium azide. |
Properties
| antibody form | purified immunoglobulin |
| clone | SPA-27 (monoclonal) |
| form | buffered aqueous solution |
| application(s) | direct ELISA: 1:80,000-1:160,000 |
| isotype | IgG1 |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| WGK Germany | 3 |
References
| reference | Berrie, E., et al., Manufacture and quality control of CAMPATH-1 antibodies for clinical trials. Cytotherapy 3, 233-242, (2001) Abstract |
